Over the course of time on Cow Chop, many guests have appeared either friends or fellow YouTubers. The following are guests that have appeared:
Micheal Jones - A member of Rooster Teeth who appeared on episode 3 of Wrong Side of Youtube: "Doop Freestyle Nightmare"
Geoff Ramsey - Also member of Rooster Teeth who appeared on episode 3 of Amazon Prime Time: "Body Bag Burial"
Dexter Manning - An old friend of the Cow Chop crew who appeared in various videos on the Cow Chop channel.
Steven Suptic - A fellow YouTuber who appeared in "Steven Suptic vs The Mouse Trap Bounce Castle" video
Khail Anonymous - A Youtuber who was featured in "Trevor Escapes to LA" but was never actually introduced.